PrimePCR™ Probe Assay: PGA5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PGA5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PGA5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041188
Assay Design:   Intron-spanning
Chromosome Location:   11:61017424-61018673question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000580994 ENST00000320362 ENST00000281513 ENST00000441750 ENST00000594671 ENST00000262960 ENST00000598800 ENST00000597849 ENST00000348261 ENST00000565831 ENST00000358590 ENST00000379589 ENST00000375581 ENST00000367338 ENST00000362061 ENST00000223210 ENST00000325558 ENST00000543125 ENST00000538258 ENST00000378149 ENST00000537932 ENST00000544899 ENST00000422676 ENST00000312403 ENST00000451616 ENST00000541528 ENST00000488633

Gene Information

Description Not Available

Gene Symbol:   PGA5
Gene Name:   pepsinogen 5, group I (pepsinogen A)
Aliases:   PGA3, PGA4
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000256713
Entrez:   5222
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.120000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download