PrimePCR™ Probe Assay: SLC44A4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC44A4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC44A4, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0041051
Assay Design:   Intron-spanning
Chromosome Location:   6:31833870-31837414question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000383379 ENST00000458130 ENST00000549677 ENST00000547684 ENST00000229729 ENST00000414427 ENST00000375562 ENST00000544672 ENST00000417894 ENST00000414684 ENST00000550401 ENST00000550821 ENST00000442152 ENST00000450933 ENST00000553121 ENST00000553147 ENST00000415517 ENST00000441920 ENST00000547493 ENST00000547288 ENST00000425238 ENST00000431710 ENST00000549663 ENST00000548188 ENST00000453831 ENST00000433046 ENST00000551168 ENST00000546461

Gene Information

The protein encoded by this gene may be a sodium-dependent transmembrane transport protein involved in the uptake of choline by cholinergic neurons. Defects in this gene can cause sialidosis a lysosomal storage disease. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq May 2010]

Gene Symbol:   SLC44A4
Gene Name:   solute carrier family 44, member 4
Aliases:   C6orf29, CTL4, FLJ14491, NG22
RefSeq:   NC_000006.11 NG_008201.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167247.1 NT_167248.1 NT_167249.1 NG_023058.1
Ensembl:   ENSG00000204385
Entrez:   80736
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.720000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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