PrimePCR™ Probe Assay: DNAJB13, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: DNAJB13, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: DNAJB13, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040978
Assay Design:   Intron-spanning
Chromosome Location:   11:73679457-73680367question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000396879 ENST00000299989 ENST00000381761 ENST00000321596 ENST00000354127 ENST00000305626 ENST00000339764 ENST00000537753 ENST00000542350 ENST00000543947

Gene Information

DNAJA13 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins. For background information on the DNAJ family see 608375.[supplied by OMIM Mar 2008]

Gene Symbol:   DNAJB13
Gene Name:   DnaJ (Hsp40) homolog, subfamily B, member 13
Aliases:   FLJ46748, RSPH16A, TSARG5, TSARG6
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000187726
Entrez:   374407
Chromosome Mapping:   11q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.620000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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