PrimePCR™ Probe Assay: RIIAD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RIIAD1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RIIAD1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040926
Assay Design:   Intron-spanning
Chromosome Location:   1:151700052-151702019question
Amplicon Length:   200
Splice Variants Targeted:   ENST00000355703 ENST00000373318 ENST00000406958 ENST00000272972 ENST00000361678 ENST00000403283 ENST00000401804 ENST00000407275 ENST00000391987 ENST00000373320 ENST00000326413 ENST00000479191 ENST00000442233 ENST00000192788 ENST00000377819 ENST00000339997 ENST00000540798 ENST00000357430 ENST00000392066 ENST00000336735 ENST00000432593

Gene Information

Description Not Available

Gene Symbol:   RIIAD1
Gene Name:   regulatory subunit of type II PKA R-subunit (RIIa) domain containing 1
Aliases:   C1orf230, FLJ36032, NCRNA00166
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000178796
Entrez:   284485
Chromosome Mapping:   1q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.460000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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