PrimePCR™ Probe Assay: GOLGA8IP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GOLGA8IP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GOLGA8IP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040815
Assay Design:   Intron-spanning
Chromosome Location:   15:23259870-23261064question
Amplicon Length:   170
Splice Variants Targeted:   ENST00000532622 ENST00000526619 ENST00000337838 ENST00000450328 ENST00000525590 ENST00000329523 ENST00000563027 ENST00000340249 ENST00000567927 ENST00000341650 ENST00000569052 ENST00000327271 ENST00000562783 ENST00000566740 ENST00000512626 ENST00000509311 ENST00000448387 ENST00000359187 ENST00000360553 ENST00000432566 ENST00000543376 ENST00000342314 ENST00000267731 ENST00000438958 ENST00000450802 ENST00000562295 ENST00000576650 ENST00000576467

Gene Information

Description Not Available

Gene Symbol:   GOLGA8IP
Gene Name:   golgin A8 family, member I, pseudogene
Aliases:   FLJ35785, GOLGA8I, GOLGA9P
RefSeq:   NC_000015.9 NT_078094.2
Ensembl:   ENSG00000153666
Entrez:   283796
Chromosome Mapping:   15q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.590000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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