PrimePCR™ Probe Assay: SCHIP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SCHIP1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCHIP1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040811
Assay Design:   Intron-spanning
Chromosome Location:   3:159609886-159614561question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000349552 ENST00000167586 ENST00000301653 ENST00000312106 ENST00000555420 ENST00000393409 ENST00000251772 ENST00000546530 ENST00000261729 ENST00000446861 ENST00000548055 ENST00000397066 ENST00000332582 ENST00000400274 ENST00000457905 ENST00000466639 ENST00000435722 ENST00000417564 ENST00000318711 ENST00000427143 ENST00000320955 ENST00000301831 ENST00000534947 ENST00000379170 ENST00000588161 ENST00000262633 ENST00000592202 ENST00000589871 ENST00000360475 ENST00000330490 ENST00000507827 ENST

Gene Information

Description Not Available

Gene Symbol:   SCHIP1
Gene Name:   schwannomin interacting protein 1
Aliases:   FLJ39160, SCHIP-1
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000151967
Entrez:   29970
Chromosome Mapping:   3q25.32-q25.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 39.460000
Efficiency 84

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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