PrimePCR™ Probe Assay: RGPD5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RGPD5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGPD5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040642
Assay Design:   Intron-spanning
Chromosome Location:   2:110559395-110561568question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000304514 ENST00000409886 ENST00000354986 ENST00000408999 ENST00000283195 ENST00000393283 ENST00000430736 ENST00000016946 ENST00000432606 ENST00000272454 ENST00000329516 ENST00000330331 ENST00000446930 ENST00000455695 ENST00000302558 ENST00000409750 ENST00000330575 ENST00000559485 ENST00000409776 ENST00000398193 ENST00000327544 ENST00000420840 ENST00000398146

Gene Information

RAN is a small GTP-binding protein of the RAS superfamily that is associated with the nuclear membrane and is thought to control a variety of cellular functions through its interactions with other proteins. This gene shares a high degree of sequence identity with RANBP2 a large RAN-binding protein localized at the cytoplasmic side of the nuclear pore complex. It is believed that this RANBP2 gene family member arose from a duplication event 3 Mb distal to RANBP2. Alternative splicing has been observed for this locus and two variants are described. Additional splicing is suggested but complete sequence for further transcripts has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   RGPD5
Gene Name:   RANBP2-like and GRIP domain containing 5
Aliases:   BS-63, BS63, DKFZp686I1842, RGP5, RGPD6
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000015568
Entrez:   84220
Chromosome Mapping:   2q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.260000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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