PrimePCR™ Probe Assay: NPIPL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NPIPL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPIPL1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040187
Assay Design:   Intron-spanning
Chromosome Location:   16:28468287-28469880question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000435324 ENST00000550983 ENST00000357796 ENST00000424293 ENST00000524087 ENST00000532298 ENST00000415833 ENST00000517539 ENST00000528249 ENST00000424340 ENST00000520915 ENST00000550665 ENST00000354563 ENST00000533640 ENST00000532254 ENST00000504841 ENST00000419180 ENST00000448012 ENST00000458643 ENST00000415645 ENST00000357370 ENST00000451409 ENST00000429990 ENST00000399147 ENST00000547494 ENST00000531598 ENST00000552140 ENST00000529166 ENST00000553201 ENST00000328085 ENST00000534799 ENST

Gene Information

Description Not Available

Gene Symbol:   NPIPL1
Gene Name:   nuclear pore complex interacting protein-like 1
Aliases:   A-575C2.4, A-761H5.5
RefSeq:   NC_000016.9 NG_023370.1 NT_010393.16
Ensembl:   ENSG00000233232
Entrez:   440350
Chromosome Mapping:   16p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.280000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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