PrimePCR™ Probe Assay: RP11-353J17.2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RP11-353J17.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RP11-353J17.2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040124
Assay Design:   Intron-spanning
Chromosome Location:   X:101715611-101716933question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000416848 ENST00000344862 ENST00000551923 ENST00000308025 ENST00000243344 ENST00000245312 ENST00000378295 ENST00000378288 ENST00000357733 ENST00000346387 ENST00000339397 ENST00000356766 ENST00000521356 ENST00000454009 ENST00000264668 ENST00000440940 ENST00000372763 ENST00000372758 ENST00000333110 ENST00000372752 ENST00000399878 ENST00000264868 ENST00000502949 ENST00000453326 ENST00000247087 ENST00000374011 ENST00000597070 ENST00000596869 ENST00000598061 ENST00000601491

Gene Information

Description Not Available

Gene Symbol:   RP11-353J17.2
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000215029
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.880000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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