PrimePCR™ Probe Assay: LY6G5C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: LY6G5C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LY6G5C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0040043
Assay Design:   Intron-spanning
Chromosome Location:   6:31644835-31647002question
Amplicon Length:   158
Splice Variants Targeted:   ENST00000450798 ENST00000324540 ENST00000551004 ENST00000418117 ENST00000449871 ENST00000548179 ENST00000375863 ENST00000383237 ENST00000375860 ENST00000375858 ENST00000458081 ENST00000432980 ENST00000550467 ENST00000455753 ENST00000435280 ENST00000549664 ENST00000431114 ENST00000411492 ENST00000549965 ENST00000449562 ENST00000383425 ENST00000548957

Gene Information

LY6G5C belongs to a cluster of leukocyte antigen-6 (LY6) genes located in the major histocompatibility complex (MHC) class III region on chromosome 6. Members of the LY6 superfamily typically contain 70 to 80 amino acids including 8 to 10 cysteines. Most LY6 proteins are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor that is directly involved in signal transduction (Mallya et al. 2002 [PubMed 12079290]).[supplied by OMIM Mar 2008]

Gene Symbol:   LY6G5C
Gene Name:   lymphocyte antigen 6 complex, locus G5C
Aliases:   C6orf20, G5C, LY6G5CA, LY6G5CB, NG33
RefSeq:   NC_000006.11 NT_007592.15 NT_113891.2 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204428
Entrez:   80741
Chromosome Mapping:   6p21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.390000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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