PrimePCR™ Probe Assay: BPY2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BPY2C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BPY2C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039936
Assay Design:   Intron-spanning
Chromosome Location:   Y:27190160-27197842question
Amplicon Length:   179
Splice Variants Targeted:   ENST00000564093 ENST00000397843 ENST00000532993 ENST00000356641 ENST00000371933 ENST00000252512 ENST00000433566 ENST00000434536 ENST00000589596 ENST00000506447 ENST00000511820 ENST00000430049 ENST00000325971 ENST00000602732 ENST00000331070 ENST00000382585 ENST00000602770 ENST00000382392 ENST00000382287 ENST00000602680 ENST00000434067 ENST00000409568 ENST00000328828 ENST00000376454 ENST00000376451 ENST00000266037 ENST00000550890 ENST00000590278

Gene Information

This gene is located in the nonrecombining portion of the Y chromosome and expressed specifically in testis. The encoded protein interacts with ubiquitin protein ligase E3A and may be involved in male germ cell development and male infertility. Three nearly identical copies of this gene exist on chromosome Y; two copies are part of a palindromic region. This record represents the more telomeric copy within the palindrome. [provided by RefSeq Jul 2008]

Gene Symbol:   BPY2C
Gene Name:   basic charge, Y-linked, 2C
Aliases:   BPY2, VCY2, VCY2C
RefSeq:   NC_000024.9 NG_004755.2 NT_011903.12
Ensembl:   ENSG00000185894
Entrez:   442868
Chromosome Mapping:   Yq11.223

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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