PrimePCR™ Probe Assay: MBD3L3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MBD3L3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MBD3L3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039900
Assay Design:   Intron-spanning
Chromosome Location:   19:7056877-7058639question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000216160 ENST00000249364 ENST00000542996 ENST00000535623 ENST00000535011 ENST00000538546 ENST00000274026 ENST00000414705 ENST00000382203 ENST00000382194 ENST00000349721 ENST00000357248 ENST00000314616 ENST00000347486 ENST00000318962 ENST00000415953 ENST00000265755 ENST00000455841 ENST00000424337 ENST00000476977 ENST00000470715 ENST00000372852 ENST00000257832 ENST00000264313 ENST00000512093 ENST00000563439 ENST00000471652 ENST00000442267 ENST00000316161 ENST00000302506 ENST00000351231 ENST

Gene Information

Description Not Available

Gene Symbol:   MBD3L3
Gene Name:   MBD3L3
Aliases:   Not Available
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000182315
Entrez:   653657
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.950000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download