PrimePCR™ Probe Assay: POTED, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POTED, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POTED, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039849
Assay Design:   Intron-spanning
Chromosome Location:   21:14983016-14987511question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000572933 ENST00000389173 ENST00000343518 ENST00000358970 ENST00000518533 ENST00000359441 ENST00000262510 ENST00000539144 ENST00000308149 ENST00000436936 ENST00000419098 ENST00000240499 ENST00000511833 ENST00000291270 ENST00000600757 ENST00000343373 ENST00000447742 ENST00000458663 ENST00000354227 ENST00000327423 ENST00000434485 ENST00000435221 ENST00000409832 ENST00000454856 ENST00000438063 ENST00000439682 ENST00000551509 ENST00000261560 ENST00000392288 ENST00000299443 ENST00000550722 ENST

Gene Information

Description Not Available

Gene Symbol:   POTED
Gene Name:   POTE ankyrin domain family, member D
Aliases:   A26B3, ANKRD21, CT104.1, POTE, POTE-21, POTE21
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000166351
Entrez:   317754
Chromosome Mapping:   21q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.070000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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