PrimePCR™ Probe Assay: CBWD5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CBWD5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CBWD5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039825
Assay Design:   Intron-spanning
Chromosome Location:   9:70484407-70486875question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000259199 ENST00000416503 ENST00000433343 ENST00000356521 ENST00000382447 ENST00000314367 ENST00000377447 ENST00000431099 ENST00000382393 ENST00000382389 ENST00000377400 ENST00000377457 ENST00000377449 ENST00000377441 ENST00000382399 ENST00000382405 ENST00000377395 ENST00000430059 ENST00000429800 ENST00000377384 ENST00000377392 ENST00000449778 ENST00000360171 ENST00000377349 ENST00000377344 ENST00000377342 ENST00000582886 ENST00000578449 ENST00000579515 ENST00000581344 ENST00000577357

Gene Information

Description Not Available

Gene Symbol:   CBWD5
Gene Name:   COBW domain containing 5
Aliases:   DC36, FLJ34856, FLJ40320, FLJ77192, MGC167043, MGC44166
RefSeq:   NC_000009.11 NT_113816.1
Ensembl:   ENSG00000147996
Entrez:   220869
Chromosome Mapping:   9q21.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.660000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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