PrimePCR™ Probe Assay: CEP170, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CEP170, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CEP170, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039821
Assay Design:   Intron-spanning
Chromosome Location:   1:243305718-243319600question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000321337 ENST00000429205 ENST00000366542 ENST00000366544 ENST00000366543 ENST00000481987 ENST00000336415 ENST00000490813 ENST00000413359 ENST00000464936 ENST00000492145

Gene Information

The product of this gene is a component of the centrosome a non-membraneous organelle that functions as the major microtubule-organizing center in animal cells. During interphase the encoded protein localizes to the sub-distal appendages of mature centrioles which are microtubule-based structures thought to help organize centrosomes. During mitosis the protein associates with spindle microtubules near the centrosomes. The protein interacts with and is phosphorylated by polo-like kinase 1 and functions in maintaining microtubule organization and cell morphology. The human genome contains a putative transcribed pseudogene. Several alternatively spliced transcript variants of this gene have been found but the full-length nature of some of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CEP170
Gene Name:   centrosomal protein 170kDa
Aliases:   FAM68A, KAB, KIAA0470
RefSeq:   NG_027811.1 NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143702
Entrez:   9859
Chromosome Mapping:   1q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.630000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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