PrimePCR™ Probe Assay: ASAH2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ASAH2C, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ASAH2C, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0039782
Assay Design:   Intron-spanning
Chromosome Location:   10:48004025-48011259question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000395526 ENST00000329428 ENST00000447815 ENST00000443575 ENST00000374007 ENST00000374006 ENST00000185907 ENST00000420079 ENST00000426610

Gene Information

Description Not Available

Gene Symbol:   ASAH2C
Gene Name:   N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2C
Aliases:   bA98I6.3
RefSeq:   NC_000010.10 NG_012763.1 NT_077571.1
Ensembl:   ENSG00000072444
Entrez:   653365
Chromosome Mapping:   10q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.690000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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