PrimePCR™ Probe Assay: ATP5L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ATP5L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ATP5L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033473
Assay Design:   Intron-spanning
Chromosome Location:   11:118272393-118277749question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000300688 ENST00000524422

Gene Information

Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled in a ratio of 3 alpha 3 beta and a single representative of the other 3. The Fo seems to have nine subunits (a b c d e f g F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq Jun 2010]

Gene Symbol:   ATP5L
Gene Name:   ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G
Aliases:   ATP5JG
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000167283
Entrez:   10632
Chromosome Mapping:   11q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.900000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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