PrimePCR™ Probe Assay: PMS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PMS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PMS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033285
Assay Design:   Intron-spanning
Chromosome Location:   2:190656579-190660585question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000441310 ENST00000409985 ENST00000409823 ENST00000374826 ENST00000424766 ENST00000447232 ENST00000420421 ENST00000418224 ENST00000392338

Gene Information

This gene encodes a protein belonging to the DNA mismatch repair mutL/hexB family. This protein is thought to be involved in the repair of DNA mismatches and it can form heterodimers with MLH1 a known DNA mismatch repair protein. Mutations in this gene cause hereditary nonpolyposis colorectal cancer type 3 (HNPCC3) either alone or in combination with mutations in other genes involved in the HNPCC phenotype which is also known as Lynch syndrome. [provided by RefSeq Jul 2008]

Gene Symbol:   PMS1
Gene Name:   PMS1 postmeiotic segregation increased 1 (S. cerevisiae)
Aliases:   DKFZp781M0253, FLJ98259, HNPCC3, PMSL1, hPMS1
RefSeq:   NC_000002.11 NG_008648.1 NT_005403.17
Ensembl:   ENSG00000064933
Entrez:   5378
Chromosome Mapping:   2q31-q33,2q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.280000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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