PrimePCR™ Probe Assay: MATR3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MATR3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MATR3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033277
Assay Design:   Intron-spanning
Chromosome Location:   5:138650364-138651808question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000509990 ENST00000506147 ENST00000504203 ENST00000502929 ENST00000394800 ENST00000509644 ENST00000394805 ENST00000512876 ENST00000513678 ENST00000504045 ENST00000510056 ENST00000503811 ENST00000514528 ENST00000515833 ENST00000361059

Gene Information

This locus encodes a nuclear matrix protein. Mutations at this locus have been associated with distal myopathy 2 which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants including read-through transcripts with an upstream locus have been described. Related pseuodgenes have been defined on chr1 and chrX.[provided by RefSeq Mar 2011]

Gene Symbol:   MATR3
Gene Name:   matrin 3
Aliases:   DKFZp686K0542, DKFZp686K23100, KIAA0723, MGC9105, MPD2, VCPDM
RefSeq:   NC_000005.9 NT_034772.6 NG_012846.1
Ensembl:   ENSG00000015479
Entrez:   9782
Chromosome Mapping:   5q31.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.970000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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