PrimePCR™ Probe Assay: TRPV1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TRPV1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRPV1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033268
Assay Design:   Intron-spanning
Chromosome Location:   17:3486639-3489143question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000399759 ENST00000399756 ENST00000425167 ENST00000310522 ENST00000174621

Gene Information

Capsaicin the main pungent ingredient in hot chili peppers elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein but with different 5' UTR sequence have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TRPV1
Gene Name:   transient receptor potential cation channel, subfamily V, member 1
Aliases:   DKFZp434K0220, VR1
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000196689
Entrez:   7442
Chromosome Mapping:   17p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.180000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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