PrimePCR™ Probe Assay: CNTNAP5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CNTNAP5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CNTNAP5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0033047
Assay Design:   Intron-spanning
Chromosome Location:   2:124979282-124999844question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000431078

Gene Information

This gene product belongs to the neurexin family members of which function in the vertebrate nervous system as cell adhesion molecules and receptors. This protein like other neurexin proteins contains epidermal growth factor repeats and laminin G domains. In addition it includes an F5/8 type C domain discoidin/neuropilin- and fibrinogen-like domains and thrombospondin N-terminal-like domains. [provided by RefSeq Jul 2008]

Gene Symbol:   CNTNAP5
Gene Name:   contactin associated protein-like 5
Aliases:   FLJ31966, caspr5
RefSeq:   NC_000002.11 NT_022135.16
Ensembl:   ENSG00000155052
Entrez:   129684
Chromosome Mapping:   2q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.900000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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