PrimePCR™ Probe Assay: SLC35C2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC35C2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC35C2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0032842
Assay Design:   Intron-spanning
Chromosome Location:   20:44979442-44980728question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000317734 ENST00000243896 ENST00000372227 ENST00000372229 ENST00000372230 ENST00000543605 ENST00000372225 ENST00000372223

Gene Information

Oxygenation levels play an important role in the regulation of cellular invasiveness which occurs during early implantation when the trophoblast cells invade the uterus as well as during tumour progression and metastasis. This gene which is regulated by oxygen tension is induced in hypoxic trophoblast cells and is overexpressed in ovarian cancer. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   SLC35C2
Gene Name:   solute carrier family 35, member C2
Aliases:   BA394O2.1, C20orf5, CGI-15, FLJ37039, FLJ46434, MGC20633, MGC32079, MGC39183, OVCOV1
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000080189
Entrez:   51006
Chromosome Mapping:   20q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 34.370000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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