PrimePCR™ Probe Assay: INPP4A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: INPP4A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: INPP4A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031433
Assay Design:   Intron-spanning
Chromosome Location:   2:99182574-99185067question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000409016 ENST00000409851 ENST00000409463 ENST00000409540 ENST00000523221 ENST00000074304 ENST00000545415

Gene Information

This gene encodes an Mg++ independent enzyme that hydrolyzes the 4-position phosphate from the inositol ring of phosphatidylinositol 34-bisphosphate inositol 134-trisphosphate and inositol 34-bisphosphate. Multiple transcript variants encoding distinct isoforms have been described. [provided by RefSeq Aug 2008]

Gene Symbol:   INPP4A
Gene Name:   inositol polyphosphate-4-phosphatase, type I, 107kDa
Aliases:   INPP4, TVAS1
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000040933
Entrez:   3631
Chromosome Mapping:   2q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.430000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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