PrimePCR™ Probe Assay: HSPB8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HSPB8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HSPB8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031389
Assay Design:   Intron-spanning
Chromosome Location:   12:119624848-119631577question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000281938

Gene Information

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells and this protein also functions as a chaperone in association with Bag3 a stimulator of macroautophagy. Thus this gene appears to be involved in regulation of cell proliferation apoptosis and carcinogenesis and mutations in this gene have been associated with different neuromuscular diseases including Charcot-Marie-Tooth disease. [provided by RefSeq Jul 2008]

Gene Symbol:   HSPB8
Gene Name:   heat shock 22kDa protein 8
Aliases:   CMT2L, DHMN2, E2IG1, H11, HMN2, HMN2A, HSP22
RefSeq:   NC_000012.11 NG_007953.1 NT_009775.17
Ensembl:   ENSG00000152137
Entrez:   26353
Chromosome Mapping:   12q24.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.993800
y-intercept 33.240000
Efficiency 109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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