PrimePCR™ Probe Assay: PDE8A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031236
Assay Design:   Intron-spanning
Chromosome Location:   15:85664177-85666345question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000310298 ENST00000339708 ENST00000394553

Gene Information

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family and PDE8 subfamily. This PDE hydrolyzes the second messenger cAMP which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus by regulating the cellular concentration of cAMP this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Jul 2011]

Gene Symbol:   PDE8A
Gene Name:   phosphodiesterase 8A
Aliases:   FLJ16150, HsT19550
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000073417
Entrez:   5151
UniGene:   Hs.9333
Chromosome Mapping:   15q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 34.390000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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