PrimePCR™ Probe Assay: MAP3K15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031231
Assay Design:   Intron-spanning
Chromosome Location:   X:19387282-19389141question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000359173 ENST00000469203 ENST00000338883

Gene Information

The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K) which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq Jul 2010]

Gene Symbol:   MAP3K15
Gene Name:   mitogen-activated protein kinase kinase kinase 15
Aliases:   ASK3, FLJ16518, bA723P2.3
RefSeq:   NG_016781.1 NC_000023.10 NG_021184.1 NT_167197.1
Ensembl:   ENSG00000180815
Entrez:   389840
UniGene:   Hs.713701
Chromosome Mapping:   Xp22.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.450000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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