PrimePCR™ Probe Assay: TMC2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031214
Assay Design:   Intron-spanning
Chromosome Location:   20:2591187-2592920question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000358864

Gene Information

This gene is considered a member of a gene family predicted to encode transmembrane proteins. The specific function of this gene is unknown; however expression in the inner ear suggests that it may be crucial for normal auditory function. Mutations in this gene may underlie hereditary disorders of balance and hearing. [provided by RefSeq Jul 2008]

Gene Symbol:   TMC2
Gene Name:   transmembrane channel-like 2
Aliases:   C20orf145, dJ686C3.3
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000149488
Entrez:   117532
UniGene:   Hs.352626
Chromosome Mapping:   20p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.780000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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