PrimePCR™ Probe Assay: CDC5L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031192
Assay Design:   Intron-spanning
Chromosome Location:   6:44355537-44358075question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000371477

Gene Information

The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC5L
Gene Name:   CDC5 cell division cycle 5-like (S. pombe)
Aliases:   CDC5, CDC5-LIKE, CEF1, KIAA0432, PCDC5RP, dJ319D22.1
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000096401
Entrez:   988
UniGene:   Hs.485471
Chromosome Mapping:   6p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.110000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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