PrimePCR™ Probe Assay: AP1G1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031181
Assay Design:   Intron-spanning
Chromosome Location:   16:71795422-71798562question
Amplicon Length:   131
Splice Variants Targeted:   ENST00000433195 ENST00000299980 ENST00000393512 ENST00000423132 ENST00000538574 ENST00000425422 ENST00000450149

Gene Information

Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha beta- beta prime- and gamma- adaptins. Adaptins together with medium and small subunits form a heterotetrameric complex called an adaptor whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   AP1G1
Gene Name:   adaptor-related protein complex 1, gamma 1 subunit
Aliases:   ADTG, CLAPG1, MGC18255
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000166747
Entrez:   164
UniGene:   Hs.461253
Chromosome Mapping:   16q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 34.770000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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