PrimePCR™ Probe Assay: CES3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031177
Assay Design:   Intron-spanning
Chromosome Location:   16:67005143-67006312question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000303334 ENST00000394037 ENST00000543856

Gene Information

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics such as cocaine and heroin and endogenous substrates with ester thioester or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism and may play a role in the blood-brain barrier system. This gene is expressed in several tissues particularly in colon trachea and in brain and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported but the biological validity and/or full-length nature of some variants have not been determined.[provided by RefSeq Jun 2010]

Gene Symbol:   CES3
Gene Name:   carboxylesterase 3
Aliases:   ES31, FLJ21736
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000172828
Entrez:   23491
UniGene:   Hs.268700
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.380000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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