PrimePCR™ Probe Assay: AGGF1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031137
Assay Design:   Intron-spanning
Chromosome Location:   5:76326774-76331401question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000312916 ENST00000506806

Gene Information

This gene encodes an angiogenic factor that promotes proliferation of endothelial cells. Mutations in this gene are associated with a susceptibility to Klippel-Trenaunay syndrome. Pseudogenes of this gene are found on chromosomes 3 4 10 and 16.[provided by RefSeq Sep 2010]

Gene Symbol:   AGGF1
Gene Name:   angiogenic factor with G patch and FHA domains 1
Aliases:   FLJ10283, GPATC7, GPATCH7, HSU84971, HUS84971, VG5Q
RefSeq:   NC_000005.9 NG_027822.1 NT_006713.15
Ensembl:   ENSG00000164252
Entrez:   55109
UniGene:   Hs.634849
Chromosome Mapping:   5q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.740000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download