This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: NR1H2, Human
PrimePCR™ Template for Probe Assay: NR1H2, Human
The liver X receptors LXRA (NR1H3; MIM 602423) and LXRB form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver adrenal gland intestine adipose tissue macrophages lung and kidney whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al. 2009 [PubMed 19436111]).[supplied by OMIM Jan 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.