PrimePCR™ Probe Assay: ARHGAP17, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ARHGAP17, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ARHGAP17, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031112
Assay Design:   Intron-spanning
Chromosome Location:   16:24953408-24955163question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000289968 ENST00000303665 ENST00000455311 ENST00000441763

Gene Information

RICH1 is a GTPase-activating protein (GAP). GAPs stimulate the intrinsic GTP hydrolysis of small G proteins such as RHOA (MIM 165390) RAC1 (MIM 602048) and CDC42 (MIM 116952).[supplied by OMIM Apr 2004]

Gene Symbol:   ARHGAP17
Gene Name:   Rho GTPase activating protein 17
Aliases:   DKFZp564A1363, FLJ37567, FLJ43368, MGC87805, MST066, MST110, MSTP038, MSTP066, MSTP110, NADRIN, PP367, PP4534, RICH1, RICH1B, WBP15
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000140750
Entrez:   55114
Chromosome Mapping:   16p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.440000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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