PrimePCR™ Probe Assay: GLCE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031080
Assay Design:   Intron-spanning
Chromosome Location:   15:69553591-69560655question
Amplicon Length:   145
Splice Variants Targeted:   ENST00000261858

Gene Information

Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal 2005 [PubMed 15853773]).[supplied by OMIM Jun 2008]

Gene Symbol:   GLCE
Gene Name:   glucuronic acid epimerase
Aliases:   HSEPI, KIAA0836
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000138604
Entrez:   26035
UniGene:   Hs.183006
Chromosome Mapping:   15q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.090000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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