PrimePCR™ Probe Assay: HAUS2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HAUS2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HAUS2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031076
Assay Design:   Intron-spanning
Chromosome Location:   15:42853517-42855989question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000260372 ENST00000391623

Gene Information

HAUS2 is 1 of 8 subunits of the 390-kD human augmin complex or HAUS complex. The augmin complex was first identified in Drosophila and its name comes from the Latin verb 'augmentare' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al. 2008 [PubMed 18443220]; Uehara et al. 2009 [PubMed 19369198]).[supplied by OMIM Jun 2010]

Gene Symbol:   HAUS2
Gene Name:   HAUS augmin-like complex, subunit 2
Aliases:   C15orf25, CEP27, FLJ10460, HsT17025
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000137814
Entrez:   55142
Chromosome Mapping:   15q15.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.780000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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