PrimePCR™ Probe Assay: TRPC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: TRPC5, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TRPC5, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031072
Assay Design:   Intron-spanning
Chromosome Location:   X:111195573-111325152question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000262839

Gene Information

This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1 TRPC3 and TRPC4. It also interacts with multiple proteins including calmodulin CABP1 enkurin Na(+)-H+ exchange regulatory factor (NHERF ) interferon-induced GTP-binding protein (MX1) ring finger protein 24 (RNF24) and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq May 2010]

Gene Symbol:   TRPC5
Gene Name:   transient receptor potential cation channel, subfamily C, member 5
Aliases:   TRP5
RefSeq:   NC_000023.10 NG_021215.1 NT_011651.17
Ensembl:   ENSG00000072315
Entrez:   7224
Chromosome Mapping:   Xq23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.080000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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