PrimePCR™ Probe Assay: SMC3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SMC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SMC3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031071
Assay Design:   Intron-spanning
Chromosome Location:   10:112335097-112337598question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000361804

Gene Information

This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular nuclear protein or a secreted protein. The nuclear form known as structural maintenance of chromosomes 3 is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan an abundant basement membrane protein. [provided by RefSeq Jul 2008]

Gene Symbol:   SMC3
Gene Name:   structural maintenance of chromosomes 3
Aliases:   BAM, BMH, CDLS3, CSPG6, HCAP, SMC3L1
RefSeq:   NC_000010.10 NG_012217.1 NT_030059.13
Ensembl:   ENSG00000108055
Entrez:   9126
Chromosome Mapping:   10q25

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.410000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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