PrimePCR™ Probe Assay: LRRC31, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031069
Assay Design:   Intron-spanning
Chromosome Location:   3:169572737-169574260question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000316428 ENST00000264676 ENST00000523069

Gene Information

Description Not Available

Gene Symbol:   LRRC31
Gene Name:   leucine rich repeat containing 31
Aliases:   FLJ23259, FLJ40019
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000114248
Entrez:   79782
UniGene:   Hs.411295
Chromosome Mapping:   3q26.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 34.730000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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