PrimePCR™ Probe Assay: NWD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031039
Assay Design:   Intron-spanning
Chromosome Location:   19:16910879-16918425question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000524140 ENST00000549814 ENST00000552788 ENST00000379808 ENST00000420818 ENST00000339803 ENST00000523826

Gene Information

Description Not Available

Gene Symbol:   NWD1
Gene Name:   NACHT and WD repeat domain containing 1
Aliases:   MGC134940
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000188039
Entrez:   284434
UniGene:   Hs.406014
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.690000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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