PrimePCR™ Probe Assay: VWA3A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031011
Assay Design:   Intron-spanning
Chromosome Location:   16:22144306-22145721question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000389398 ENST00000310694 ENST00000299840 ENST00000389397

Gene Information

Description Not Available

Gene Symbol:   VWA3A
Gene Name:   von Willebrand factor A domain containing 3A
Aliases:   FLJ40941, FLJ46765
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000175267
Entrez:   146177
UniGene:   Hs.10697
Chromosome Mapping:   16p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.100000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download