PrimePCR™ Probe Assay: NAA38, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0031009
Assay Design:   Intron-spanning
Chromosome Location:   7:117825708-117828422question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000249299 ENST00000424702 ENST00000422760 ENST00000411938

Gene Information

This gene encodes a member of the like-Sm family of proteins. The encoded protein consists of a closed barrel shape made up of five anti-parallel beta strands and an alpha helix. This protein partners with six paralogs to form a heteroheptameric ring which transiently binds U6 small nuclear RNAs and is involved in the general maturation of RNA in the nucleus. [provided by RefSeq Jan 2010]

Gene Symbol:   NAA38
Gene Name:   N(alpha)-acetyltransferase 38, NatC auxiliary subunit
Aliases:   FLJ33242, LSM8
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000128534
Entrez:   51691
UniGene:   Hs.655046
Chromosome Mapping:   7q31.1-q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.880000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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