PrimePCR™ Probe Assay: TMEM159, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030977
Assay Design:   Intron-spanning
Chromosome Location:   16:21185364-21190843question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000233047 ENST00000261388 ENST00000451578

Gene Information

Description Not Available

Gene Symbol:   TMEM159
Gene Name:   transmembrane protein 159
Aliases:   PROMETHIN
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000011638
Entrez:   57146
UniGene:   Hs.258212
Chromosome Mapping:   16p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.960000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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