PrimePCR™ Probe Assay: CAPN14, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030954
Assay Design:   Intron-spanning
Chromosome Location:   2:31417302-31422341question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000403897 ENST00000444918

Gene Information

Calpains are a family of cytosolic calcium-activated cysteine proteases involved in a variety of cellular processes including apoptosis cell division modulation of integrin-cytoskeletal interactions and synaptic plasticity (Dear et al. 2000 [PubMed 10964513]). CAPN14 belongs to the calpain large subunit family.[supplied by OMIM Mar 2008]

Gene Symbol:   CAPN14
Gene Name:   calpain 14
Aliases:   FLJ54788
RefSeq:   NC_000002.11 NT_022184.15
Ensembl:   ENSG00000214711
Entrez:   440854
UniGene:   Hs.468059
Chromosome Mapping:   2p23.1-p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.050000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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