PrimePCR™ Probe Assay: GRIN2D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030924
Assay Design:   Intron-spanning
Chromosome Location:   19:48908506-48917286question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000263269

Gene Information

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA channel has been shown to be involved in long-term potentiation an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A) NMDAR2B (GRIN2B) NMDAR2C (GRIN2C) and NMDAR2D (GRIN2D). [provided by RefSeq Mar 2010]

Gene Symbol:   GRIN2D
Gene Name:   glutamate receptor, ionotropic, N-methyl D-aspartate 2D
Aliases:   EB11, NMDAR2D, NR2D
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105464
Entrez:   2906
UniGene:   Hs.445015
Chromosome Mapping:   19q13.1-qter

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.530000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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