PrimePCR™ Probe Assay: CHAF1A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: CHAF1A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CHAF1A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030919
Assay Design:   Intron-spanning
Chromosome Location:   19:4402780-4408982question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000344143 ENST00000535117 ENST00000301280

Gene Information

Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50 p60 (CHAF1B; MIM 601245) and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al. 1995 [PubMed 7600578]).[supplied by OMIM Mar 2008]

Gene Symbol:   CHAF1A
Gene Name:   chromatin assembly factor 1, subunit A (p150)
Aliases:   CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
RefSeq:   NC_000019.9 NG_028084.1 NT_011255.14
Ensembl:   ENSG00000167670
Entrez:   10036
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.120000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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