PrimePCR™ Probe Assay: TMC5, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030904
Assay Design:   Intron-spanning
Chromosome Location:   16:19475250-19477471question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000381414 ENST00000219821 ENST00000541464 ENST00000396229 ENST00000542583 ENST00000440743

Gene Information

Description Not Available

Gene Symbol:   TMC5
Gene Name:   transmembrane channel-like 5
Aliases:   FLJ13593
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000103534
Entrez:   79838
UniGene:   Hs.115838
Chromosome Mapping:   16p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.700000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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