PrimePCR™ Probe Assay: TJP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030881
Assay Design:   Intron-spanning
Chromosome Location:   19:3730651-3732009question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000262968 ENST00000541714 ENST00000382008 ENST00000539908

Gene Information

TJP3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al. 1999 [PubMed 10601346]).[supplied by OMIM Mar 2009]

Gene Symbol:   TJP3
Gene Name:   tight junction protein 3 (zona occludens 3)
Aliases:   MGC119546, ZO-3, ZO3
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000105289
Entrez:   27134
UniGene:   Hs.25527
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 34.800000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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