PrimePCR™ Probe Assay: ELK4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030872
Assay Design:   Intron-spanning
Chromosome Location:   1:205589909-205592876question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000357992 ENST00000289703 ENST00000539916

Gene Information

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is phosphorylated by the kinases MAPK1 and MAPK8. Several transcript variants have been described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ELK4
Gene Name:   ELK4, ETS-domain protein (SRF accessory protein 1)
Aliases:   SAP1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000158711
Entrez:   2005
UniGene:   Hs.497520
Chromosome Mapping:   1q32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.950000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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