PrimePCR™ Probe Assay: AC011443.1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: AC011443.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: AC011443.1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030865
Assay Design:   Intron-spanning
Chromosome Location:   19:39592123-39597665question
Amplicon Length:   104
Splice Variants Targeted:   ENST00000331256

Gene Information

Purple acid phosphatases (PAPs) including PAPL are a family of binuclear metallohydrolases that have been identified in plants animals and fungi (Flanagan et al. 2006 [PubMed 16793224]).[supplied by OMIM Mar 2008]

Gene Symbol:   AC011443.1
Gene Name:   Iron/zinc purple acid phosphatase-like protein
Aliases:   FLJ16165
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000183760
Entrez:   390928
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.090000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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