PrimePCR™ Probe Assay: SGTA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0030856
Assay Design:   Intron-spanning
Chromosome Location:   19:2763709-2767145question
Amplicon Length:   128
Splice Variants Targeted:   ENST00000221566

Gene Information

This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however its function is not known. Since this transcript is expressed ubiquitously in various tissues this protein may serve a housekeeping function. [provided by RefSeq Jul 2008]

Gene Symbol:   SGTA
Gene Name:   small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Aliases:   SGT, alphaSGT, hSGT
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000104969
Entrez:   6449
UniGene:   Hs.203910
Chromosome Mapping:   19p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.960000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download